Fig 2: 2′-O-methylation at predicted SNORD113-6/AF357425 binding sites. For the detection of 2′-O-methyated nucleotides, RTL-Q was performed. PMFs or HUAFs were transfected with GM-ctrl or GMs against murine AF357425 (GM-AF) or human SNORD113-6 (GM-113) for 24 h. The EMF was calculated and shown as part of the relative gene expression. Relative expression and EMF of murine targets (A) Itgb3, (B) Map2k1, (C) Itga7, (D) Ntn4, (E) Jag1 and (F) Dusp7 in PMFs transfected with GM-ctrl or GM-AF. Relative expression and EMF of human targets (G) pre-MAP2K1, (H) PARVB, (I) ITGA7, (J) FLNB, (K) pre-NTN4, (L) DUSP7 and (M) pre-EBPL in HUAFs transfected with GM-ctrl or GM-113. Expression levels are relative to U6. Data are represented as mean ± SEM.

Fig 3: Protein expression in murine and human primary fibroblasts. (A) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, each with GAPDH, of three independent replicates (each band represents a pool of three technical triplicates) in PMFs. (B) Western blots for JAG1, DUSP7, ITGB3 and MAP2K1, with GAPDH of three independent replicates (each band represents a pool of three technical triplicates) in HUAFs. (C–L) Quantifications of protein band intensities, normalized to GAPDH. Data are represented as mean ± SEM. **P < 0.01, compared with GM-ctrl.